The Comic Relief single Islands in the Stream, performed by Gavin and Stacey stars Rob Brydon and Ruth Jones, has taken over the UK number one spot.
Its success adds to the £5.5m raised by Comic Relief singles over two decades.
The Saturday's version of Just Can't Get Enough, also in aid of Comic Relief, is at number three.
U2's album, No Line on the Horizon, remains top of the album chart, holding off a greatest hits collection by Scottish singer Annie Lennox.
American Idol winner Kelly Clarkson had a new entry in the album chart at number three with All I Ever Wanted, which features her recent chart-topping single My Life Would Suck Without You.

UK TOP FIVE SINGLES

• 1 Islands in the Stream - Comic Relief
• 2 Right Round - Flo Rida
• 3 Just Can't Get Enough - The Saturdays
• 4 Poker Face - Lady GaGa
• 5 Love Story - Taylor Swift (pictured)

Source: Official Charts Company

Other new entries in the album chart included US country singer Taylor Swift at number five with Fearless, followed at six by 13-year-old Faryl Smith, a finalist on Britain's Got Talent, with her eponymous debut.
Islands in the Stream is Comic Relief's 11th number one single, following in the footsteps of Peter Kay's Is This the Way to Amarillo, Westlife's Uptown Girl and Boyzone's When the Going Gets Tough.
Last week's number one, Right Round by Flo Rida, slipped to number two, with Lady GaGa at four with Poker Face and Swift making up the top five with Love Story.